Molecular biology techniques : a classroom laboratory manual
Carson, Susan / Miller, Heather B.
Fourth edition
2023
1 ressource en ligne (xxiv, 270 pages)
Format PDF
Advanced Basic Science
Anglais
1. Getting Oriented; Practicing With Micropipettes -- 2. Purification and Digestion of Plasmid (Vector) DNA -- 3. Completion of Vector Preparation and Polymerase Chain Reaction Amplification of egfp -- 4. Preparation of Insert DNA (egfp) PCR Product -- 5. DNA Ligation and Transformation of Escherichia coli Advanced Alternatives Within Part II -- 6. Screening of Transformants, Part I -- 7. Screening of Transformants, Part II -- 8. Analysis of DNA Sequence From a Positive Clone, Part II Advanced Alternatives Within Part III -- 9. Expression of Fusion Protein from Positive Clones, SDS_PAGE and Western Blot: Part I -- 10. Expression of Fusion Protein from Positive Clones, SDS_PAGE, and Western Blot: Part II -- 11. Extraction of Recombinant Protein From Escherichia coli Using a Glutathione Affinity Column -- 1-- 2. Analysis of Purification Fractions -- 13. Total RNA Purification -- 14. Analysis of gst::egfp mRNA Levels by RT-qPCR: Part I -- 15. Analysis of gst::egfp mRNA Levels by RT-qPCR: Part II -- 16. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part I -- 17. Analysis of gst::egfp mRNA Levels by Semiquantitative RT-PCR: Part II -- 18. Culturing Mammalian Cells -- 19. Transient Transfection of Mammalian Cells -- 20. RNAi-Mediated Knockdown of EGFP: Part I -- 21. RNAi-Mediated Knockdown of EGFP: Part II -- 22. RNAi-Mediated Knockdown of EGFP: Part III -- 23. CRISPR-Mediated Knockout of EGFP: Part I -- 24. CRISPR-Mediated Knockout of EGFP: Part II -- 25. Advanced CRISPR: Part I -- 26. Advanced CRISPR: Part II -- 27. Interim Laboratory Session -- 28. Advanced CRISPR: Part IV -- 29. Advanced CRISPR: Part V -- 30. Advanced CRISPR: Part VI
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein.
Biologie moléculaire - Méthodologie / Génétique - Technique